ABSTRACT
<p><b>OBJECTIVE</b>To study the protection of puerarin on the cerebral injury in the rats with acute local ischemia.</p><p><b>METHOD</b>Rat was evaluated model of acute local cerebral ischemia was made by ligating middle cerebral artery. The cerebral damage toxylin and eosin((HE).</p><p><b>RESULT</b>The number of died neurons were significantly less in puerarin-treated rats than in the rats with cerebral ischemia (P < 0. 05). Similarly, the cerebral edema were significantly attenuated in the puerarin-treated rats as compared with cerebrally ischemic rats.</p><p><b>CONCLUSION</b>Puerarin can prevent the neuron from damage induced by acute cerebral ischemia.</p>
Subject(s)
Animals , Female , Male , Rats , Brain Ischemia , Pathology , Cell Death , Cerebral Cortex , Pathology , Infarction, Middle Cerebral Artery , Pathology , Isoflavones , Pharmacology , Neurons , Neuroprotective Agents , Pharmacology , Rats, Wistar , Vasodilator Agents , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To study the effect of puerarin on the expression of Hsp (heat shock protein) 70 in the rats with cerebral injury induced by acute local ischemia.</p><p><b>METHOD</b>Rat model of acute local cerebral ischemia was made by ligating middle cerebral artery. The Hsp70 expression in brain tissue was detected by SP method of immunohistochemistry.</p><p><b>RESULT</b>The expression of Hsp70 was significantly higher in puerarintreated rats than those in the rats with cerebral ischemia.</p><p><b>CONCLUSION</b>Puerarin can enhance the level of Hsp70 expression in the rats with cerebral injury induced by acute local ischemia.</p>
Subject(s)
Animals , Female , Male , Rats , Brain Ischemia , Metabolism , Pathology , HSP70 Heat-Shock Proteins , Metabolism , Infarction, Middle Cerebral Artery , Isoflavones , Pharmacology , Neurons , Metabolism , Neuroprotective Agents , Pharmacology , Plants, Medicinal , Chemistry , Pueraria , Chemistry , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Puerarin Injection on the hemorheology in acute blood-stasis model rats.</p><p><b>METHOD</b>The acute blood-stasis model rats were made by being soaked in ice water afer being injected adrenaline hydrochloride injection in a major dose. The changes of viscosity of whole blood and plasma, blood yield stress, erythrocyte aggregation and the maximum rate of platelet aggregation in the acute blood-stasis model rats were measured with Auto-Viscometer, and then the influence of Puerarin Injection on the hemorheology in the model rats was investigated.</p><p><b>RESULT</b>The viscosity of whole blood and plasma, and blood yield stress in the acute blood-stasis model rats were significantly higher than those in the normal control group (P < 0.01, P < 0.05). Both the high dose and the low dose of Puerarin Injection could reduce the viscosity of whole blood and plasma, blood yield stress and the maximum rate of platelet aggregation in the acute blood-stasis model rats (P < 0.01, P < 0.05). The high dose could also reduce the erythrocyte aggregation and the deformed Index of red blood cell (P < 0.05).</p><p><b>CONCLUSION</b>Puerarin Injection can ameliorate the hemorheology in acute blood-stasis model rats, and it has a dose-response relationship.</p>